Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy.
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چکیده
منابع مشابه
Centriole cycle in Chinese hamster ovary cells as determined by whole- mount electron microscopy
In interphase Chinese hamster ovary (CHO) cells, the centrosome is attached to the nucleus very firmly. This nuclear-centrosome complex is isolated as a coherent structure by lysis and extraction of cells with Triton X-100 in a low ionic strength medium. Under these conditions, the ultrastructure of the centrioles attached to the nucleus can be discerned by electron microscopy of whole-mount pr...
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During bipolar mitosis a pair of centrioles is distributed to each cell but the activities of the two centrioles within the pair are not equivalent. The parent is normally surrounded by a cloud of pericentriolar material that serves as a microtubule-organizing center. The daughter does not become associated with pericentriolar material until it becomes a parent in the next cell cycle (Rieder, C...
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The structural changes in the centrioles in Chinese hamster ovary cells were monitored by electron microscopy of whole mount preparations to investigate the effects of colcemid on the events in the centriole cycle. The population of mitotic cells increased with time of incubation with colcemid, but the arrest at mitosis by this drug was soon overcome, resulting in the formation of nuclei and a ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1981
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.91.3.814